Summary
Type: SPE
Application #: 304740
Catalog#: 730952 - Chromab. Columns HR-XC, 3 ml, 200 mg
Detection:
Conditions: Column conditioning: 5 mL methanol, then 5 mL 2 % NH3
(Do not let run the column dry!)
Sample aspiration: The prepared sample is passed through the column by vacuum.
Washing: With the following washing mixtures impurities are removed:
a) 2 mL 2 % NH3
b) 2 mL 30 % methanol / 5 % NH3
c) 2 mL 0.1 N HCl
d) 2 mL methanol
Drying: With nitrogen or air
Elution: Analytes are eluted with 4 mL methanol / 5 % NH3
Evaporation to dryness and reconstitution with 1 mL of mobile phase from subsequent HPLC
Authors: SPE department
Source: Macherey-Nagel